Jennifer Geddes-McAlister

Department
Email Address
[email protected]
Research Areas
Research Keywords
Research Description

The Geddes-McAlister lab investigates interactions between microbial pathogens and the host during infection to uncover novel therapeutic treatment options. By exploring the proteins produced under specific growth conditions, we can identify and characterize novel infection-associated proteins and understand how they target host cells to promote disease progression.

Research Summary

We are interested in characterizing the mechanisms of pathogenesis, adaptation, and survival in fungal and bacterial microbes from a systems biology perspective through mass spectrometry-based quantitative proteomics. Specifically, research in the lab centres around the following areas:
1. Systems biology to elucidate microbial proteome dynamics and interactions;
2. Mechanistic characterization of pathogenic proteins; and
3. Mass spectrometry-based proteomics for drug discovery and repurposing.
Our long-term goals emphasize the utilization of mass spectrometry-based proteomics to fundamentally understand differential microbial adaptation and survival strategies and to integrate a novel systems biology platform for investigation of diverse biological systems.

Techniques Used

Quantitative proteomics, protein extraction and quantification, peptide purification, mammalian cell culture, microbial cell culture, western blotting, ELISA assays, gene deletion, PCR, gel electrophoresis, protein purification, site-directed mutagenesis.

Lab Equipment

Probe sonicator, tissue culture, biosafety cabinets, speed-vac, gel-doc, western blotting equipment, thermal shaker, PCR, centrifugation, cell counting, electroporator, incubators

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