Wei Zhang

Department
Email Address
[email protected]
Research Areas
Research Keywords
Research Description

My research team creates synthetic probes modulating protein-protein interactions to: 1) accelerate understanding of biology, and 2) facilitate development of novel therapeutics.

Research Summary

First, we have systematically generated inhibitors and activators for E3 ubiquitin ligases to discover new enzyme catalytic mechanism and new substrates. We continue to develop synthetic peptides and proteins to delineate biochemical mechanisms of E3 ubiquitin ligases.
Second, we showed that structure-based protein engineering enables development of anti-viral reagents for Middle East respiratory syndrome (MERS) coronavirus. Now we started engineering post-translational modifications to probe and rewire DNA damage signaling for cancer therapeutics.
Finally, we created molecular tools to increase CRISPR-Cas9 genome-editing efficiency. Now we are developing new tools as "off-switch" for CRISPR-based gene editing through targeted protein degradation.

Techniques Used

Protein expression and purification, Protein design and engineering, ELISA, SPR, Microscopy, Flow cytometry, DNA damage sensitivity, CRISPR, Gene editing

Lab Equipment

FPLC, Plate Reader and Washer, SPR, Western Blotting, Tissue Culture

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