Keyword: Enzymology

• Molecular and Cellular Biology

For bacteria, survival requires evading detection. Pathogens must evade their host, but all bacteria need to avoid being targeted by phages. Gram negative bacteria’s survival depends on lipopolysaccharide and capsule – highly complex carbohydrate molecules that coat their outer surface. The enzymes that produce these molecules are complex, drawing on a large set of basic modules but then tweaking and combining them into new organizations that accomplish unique ends. My lab is focused on understanding how the structures and large-scale architectures of these enzymes create the enormous variety of unique custom carbohydrates observed in nature. To this end, we use crystallography, enzymology, and a variety of biophysical assays and bioinformatics tools to better understand these proteins.