Graham Holloway

Email Address
[email protected]
Research Description

In my lab, we study how fatty acids are broken down in skeletal and cardiac muscle. We apply this knowledge to the understanding of human exercise performance as well as diseases such as type 2 diabetes and heart failure.

Research Summary

My research is primarily focused on understanding the regulation of mitochondrial bioenergetics, with a particular interest in studying fatty acid oxidation (breakdown of fat yielding energy) in skeletal and cardiac muscle. I use a variety of techniques to examine mitochondrial function (isolated mitochondria, permeabilized fibres, whole muscle incubations), use molecular biological approaches to up-and down-regulate mitochondrial proteins, as well exercise, altered nutrition and aerobic training to study novel regulation in mitochondrial bioenergetics. We apply basic knowledge garnered from these studies to the study of human exercise performance as well as type 2 diabetes, heart failure, diabetic cardiomyopathy and various neuropathologies, conditions that have all been affiliated with alterations in mitochondria as a key event in the progression and/or development of the disease.

Techniques Used

To examine mitochondrial function, we use isolated mitochondria, permeabilized fibres, and whole muscle incubations. In addition, we use molecular biological approaches to up-and down-regulate mitochondrial proteins, as well exercise, altered nutrition and aerobic training to study novel regulation in mitochondrial bioenergetics.

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