Teresa Crease

Email Address
[email protected]
Research Description

concerted evolution, rDNA copy number, ribosomal protein genes, transposition rate, transposons, Daphnia, breeding system variation

Research Summary

Research in the Crease lab uses freshwater crustaceans in the genus Daphnia as a model organism to study evolution of the ribosomal (r)DNA multigene family, and of the DNA transposon, Pokey, which inserts in a specific region of the Daphnia rDNA repeat as well as other genomic locations. Current projects involve comparing rates of evolution in ribosomal proteins that bind to conserved and variable regions of rRNA genes, determining the impact of breeding system (cyclic or obligate parthenogenesis) on the evolution of rDNA and Pokey transposons, determining the relationship between rDNA copy number and Pokey distribution, and measuring rates of Pokey transposition inside and outside of rDNA.

Techniques Used

DNA extraction, PCR, quantitative PCR, DNA sequencing, molecular cloning

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